A practical guide to DNA-based methods for biodiversity assessment. Advanced Books. https://doi.org/10.3897/ab.e68634

Author: Bruce Kat, Blackman Rosetta, Bourlat Sarah J., Hellström Ann Micaela, Bakker Judith, Bista Iliana, Bohmann Kristine, Bouchez Agnès, Brys Rein, Clark Katie, Elbrecht Vasco, Fazi Stefano, Fonseca Vera, Hänfling Bernd, Leese Florian, Machler Elvira, Mahon Andrew, Meissner Kristian, Panksep Kristel, Pawlowski Jan, Yáñez Paul Schmidt, seymour mathew, Thalinger Bettina, Valentini Alice, Woodcock Paul, Traugott Michael, Vasselon Valentin, Deiner Krist
Publisher: on date and placePensoft Publishers, Sofia, 2021-12-02

ABOUT BOOK

This document aims to summarise the scientific consensus relating to every step of the field and laboratory workflows involved in the most common types of samples and analyses. We do not go into great detail regarding bioinformatics (computational processing of sequence data) and data analysis since these are extensive topics in their own right. We uniquely set the field and lab steps in the context of the practical and logistical constraints faced by environmental managers in terms of cost, logistics, safety, ease-of-use, and quality assurance, highlighting key decisions to be made and the inherent trade-offs associated with the various options. We hope that this will support non-experts, and those new to the field, to navigate the key considerations associated with planning or evaluating monitoring programmes using DNA-based monitoring methods. Additionally, it will aid decision-makers in writing and evaluating tenders and proposals, ensuring that the methods used for a given project are fit-for-purpose and that results are correctly interpreted.

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